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Cover for Regulation of Angiotensin Type 1 Receptor Function by an Upstream Short Open Reading Frame (sORF) in the mRNA 5' Leader Sequence
dc.contributor.advisorSandberg, Kathryn
dc.creator
dc.date.accessioned2020-01-07T15:08:03Z
dc.date.created2019
dc.date.issued
dc.date.submitted01/01/2019
dc.identifier.uri
dc.descriptionPh.D.
dc.description.abstractThe angiotensin type 1 receptor (AT1R) plays a critical role in blood pressure control. In this study, we investigated the role of an upstream short open reading frame (sORF) in AT1aR signaling. We investigated Angiotensin II (Ang II)- and Sar1,Ile4,Ile8-Ang II (SII)-induced extracellular signal regulated kinases 1 and 2 (ERK1/2) signaling and its alteration in the presence of an sORF in exon (E) 2 in the AT1aR mRNA 5’ leader sequence (LS). Human embryonic kidney 293 cells (HEK293) were transfected with WT [E1,2,3-AT1aR-EGFP] or MT [E1,2(-108T),3-AT1aR-EGFP]. A single nucleotide was mutated at the start codon of the intact sORF (WT) to create a disrupted sORF (MT). Dose response curves for ERK1/2 activation in WT vs MT were significantly different for Ang II (p
dc.formatPDF
dc.format.extent125 leaves
dc.languageen
dc.publisherGeorgetown University
dc.sourceGeorgetown University-Graduate School of Arts & Sciences
dc.sourceBiochemistry & Molecular
dc.subjectAngiotensin II
dc.subjectAngiotensin type 1 receptor
dc.subjectApoptosis
dc.subjectERK1/2
dc.subject[Sar1-Ile4-Ile8]-Ang II
dc.subjectshort open reading frame
dc.subject.lcshBiochemistry
dc.subject.lcshCytology
dc.subject.otherBiochemistry
dc.subject.otherCellular biology
dc.titleRegulation of Angiotensin Type 1 Receptor Function by an Upstream Short Open Reading Frame (sORF) in the mRNA 5' Leader Sequence
dc.typethesis
gu.embargo.lift-date2021-01-07
gu.embargo.termscommon-1-year


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