Browsing Graduate Theses and Dissertations - Biochemistry and Molecular & Cellular Biology by Creation Date
Now showing items 21-40 of 67
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p15Ink4b Loss Impairs the Maturation and Activation of Conventional Dendritic Cells
(Georgetown University, 2012)p15Ink4b, an inhibitor of cyclin-dependent kinases, is a tumor suppressor frequently associated with hematological malignancies. Its inactivation through DNA methylation is one of the most prevalent epigenetic alterations ... -
Histone ADP-ribosylation by Poly(ADP-ribose) Polymerase 1 (PARP1) Facilitates Inflammatory Gene Transcription by Directly Altering Nucleosome Structure
(Georgetown University, 2012)The packaging of DNA into nucleosomes imposes obstacles to gene transcription as they limit the accessibility of DNA to the transcription machinery. As such, the modulation of nucleosome structure by histone-modifying and ... -
Role of the Ubiquitin Proteasome System in the Regulation of V(D)J Recombination
(Georgetown University, 2012)V(D)J recombination, the only known site specific DNA rearrangement in vertebrates, is the process that is responsible for the development of vertebrate adaptive immunity. It mediates the assembly of antigen receptor genes ... -
NOVEL ROLES FOR IRON-SULFUR CLUSTERS IN RED BLOOD CELL DEVELOPMENT AND HUMAN DISEASE
(Georgetown University, 2012)Iron-sulfur (Fe-S) clusters are versatile enzyme cofactors that are essential to all living organisms due to their diverse electron transfer and catalytic capabilities. In this collection of studies, we investigated the ... -
Cis and Trans Regulation of the ANK1 Locus
(Georgetown University, 2012)Many mammalian loci have alternate promoters in which transcripts contain distinct first exons that are fused to a common exon 2 in different cell types. The human ankyrin 1 gene (ANK1) is a classic example of this type ... -
ALTERATION OF MAMMARY GLAND DEVELOPMENT AND GENE EXPRESSION BY IN UTERO EXPOSURE TO METALLOESTROGENS
(Georgetown University, 2012)Early life exposure to estrogens and estrogen like contaminants in the environment are thought to increase the risk of developing breast cancer due to the early onset of puberty in the exposed female. However, the results ... -
Analysis of Plasmodium falciparum Chloroquine Resistance Transporters in Saccharomyces cerevisiae
(Georgetown University, 2012)The evolution of drug resistance in malaria parasites continues to hamper global eradication campaigns and wreak havoc in endemic countries. Mutations in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) ... -
Acidic phospholipid deficiency results in an inhibition of chromosomal replication and cell-cycle specific arrest in Escherichia coli
(Georgetown University, 2012)Beyond the simplistic function as an environmental barrier, lipids have a variety of functions. The wealth of knowledge available on Escherichia coli (E. coli), and its small subset of lipid species, makes it an ideal ... -
THE CX3CR1+ LAMINA PROPRIA DENDRITIC CELL: MASTER SENTINEL OF THE TERMINAL ILEUM
(Georgetown University, 2012)The CX3CR1+ lamina propria dendritic cell (LPDC) of the terminal ileum provides a unique sentinel role, critical for intestinal health. Here, we demonstrate that three Crohn's disease associated single nucleotide polymorphisms ... -
ROLE OF BTG2 IN THE ANTIOXIDANT RESPONSE IN HUMAN BREAST EPITHELIAL CELLS.
(Georgetown University, 2012)BTG2, a member of antiproliferative gene family BTG/TOB, has been implicated in cell cycle regulation, normal development and tumor suppression. However, the underlying mechanism(s) by which BTG2 regulates these processes ... -
Modulation of dopamine transporter trafficking by the synuclein family of proteins
(Georgetown University, 2013)Dopamine signaling is controlled in part by pre-synaptic re-uptake of dopamine through the dopamine transporter (DAT), and is therefore regulated by the distribution of DAT to the cell surface. DAT trafficking is modulated ... -
Inhibition of Prostate Cancer Invasion by Small Molecule Antagonists of ERG and ETV1 Transcription Factors
(Georgetown University, 2013)Chromosomal translocations involving the ETS family of transcription factors are found in a majority of prostate cancers, including the most clinically aggressive forms. These translocations produce a chimeric gene, which ... -
Multiple, Unexpected Roles of the Antisense RNA McaS: Implications for the Prediction of sRNA Function
(Georgetown University, 2013)In the last decade, our understanding of bacterial gene regulation has undergone a dramatic shift, as small RNA (sRNA) regulators are increasingly understood to be key components of complex regulatory networks controlling ... -
Characterization of Inter-alpha-Trypsin Inhibitor Heavy Chain H4 Glycoprotein Microheterogeneity using Glycopeptide CID Tandem Mass Spectra and Glycan Database Searching
(Georgetown University, 2013)Collision-induced dissociation tandem mass spectrometry (CID MS/MS) is an effective tool for site-specific analysis of glycopeptides and a powerful means of studying glycoprotein microheterogeneity. However, manual ... -
"Examining TGF-beta Inhibition, via Smad7, and the Role of The Inhibitors of Differentiation (Id) in Metastatic Melanoma"
(Georgetown University, 2013)TGF-β, Ids and tumorigenesis are interrelated. Smad7 (S7) has been shown to potently suppress melanomagenesis through downregulation of metastasis-related genes such as MMP2, MMP9, osteopontin and CXCR4. Here, we ... -
The Effect of Sequence Variation on the Essential Protein Interactions of Pathogens - a Computational Analysis
(Georgetown University, 2014)ABSTRACT -
ID3 INDUCES AN ELK-1- AND CASPASE-8-DEPENDENT APOPTOTIC PATHWAY IN SQUAMOUS CARCINOMA CELLS
(Georgetown University, 2014)Inhibitors of differentiation/DNA binding (Id) proteins are helix-loop-helix (HLH) transcription factors. The Id protein family (Id1-Id4) mediates tissue homeostasis by regulating cellular processes including differentiation, ... -
Mahanine Disrupts Androgen Receptor Signaling in Human Prostate Cancer Cells
(Georgetown University, 2014)Continued reliance on androgen receptor (AR) signaling is an established hallmark of prostate cancer and castration-resistant prostate cancer (CRPC). Recent findings implicate the androgen receptor splice variant, ARv7 in ... -
DESIGN AND SYNTHESIS OF SMALL MOLECULE DISRUPTORS OF EWS-FLI1 IN EWING'S SARCOMA
(Georgetown University, 2014)Ewing's sarcoma (ES) consists of highly malignant tumors of the bone and soft tissues. Ninety-five percent of ES cases contain a balanced t(11;22)(q24;q12) translocation, combining the amino-terminus of EWS to the ... -
α-SYNUCLEIN DISRUPTS INTEGRATED SIGNALING BY THE UNFOLDED PROTEIN RESPONSE THROUGH INHIBITION OF ATF6-α INCORPORATION INTO COPII VESICLES
(Georgetown University, 2015)Parkinson's Disease (PD) is a neurodegenerative disease characterized by the advanced loss of motor functions as a result of progressive degenerative conditions that develop in many regions of the brain and manifest as a ...
