METAL ION-MEDIATED INACTIVATION AND SMALL MOLECULE INHIBITION OF THE METALLOPROTEASE IN BOTULINUM NEUROTOXIN A
Advisor
Yang, David C.H.
Abstract
Botulinum neurotoxins are the causative agents of botulism and serotype A (BoNT/A) is the most persistent one among seven Clostridium botulinum serotypes. Current treatments for BoNT/A toxicity do not meet the demand of a potential mass outbreak of botulism. The catalytic domain of BoNT/A light chain (LC) is the main target of the inhibitor designs.
The inhibition and binding data of quinolinol inhibitors suggest that the inhibition of NSC 84096 is likely due to the binding around the active site pocket rather than removing the catalytic Zn2+ of LC/A. NSC 84096 was successfully docked to the active site of LC/A with and without Zn2+. Based on the structural information of known LC/A inhibitors, about 400,000 compounds in the ZINC database were screened by eHiTS-LASSO. everal new scaffolds and potential leads were obtained.
Other than small organic compounds, Zn2+ and Cu2 showed strong inhibition of LC/A activity at micromolar concentrations, implicating that the inhibition is likely associated with specific binding to LC/A. The inhibition of LC/A cysteine and histidine mutants by Zn2+ and the inactivation of LC/A by thiol-selective reagent N-ethylmaleimide suggest that C165 is the likely transition metal binding site.
More than only inhibiting LC/A, Cu2+ was able to inactivate LC/A through Fenton-like reaction in the presence of ascorbate or hydrogen peroxide. Cu2+ effectively inactivated LC/A protease activity at submicromolar concentrations in the presence of both hydrogen peroxide and ascorbate. Cu2+ mediated inactivation of LC/A was time-dependent and irreversible. The second order rate constant of the inactivation of LC/A by Cu2+ was found to be 4.24 ± 0.26 µM-1 min-1.
The Cu2+-mediated inactivation of LC/A was not affected by the addition of the known LC/A inhibitor NSC 84096, which is a quinolinol derivative. NSC 84096 and similar quinolinol inhibitors in combination with Cu2+ could potentially deliver the inhibitors across cell membrane through chelation and subsequently inactivate BoNT/A.
Due to the several-month long persistence of the BoNT/A toxicity, irreversible inactivation may offer a more effective therapy than conventional inhibitors. The Cu2+ mediated inactivation of LC/A developed in the present studies is applicable to similar Cu2+ binding proteins and nucleic acids.
Description
Ph.D.
Permanent Link
http://hdl.handle.net/10822/558231Date Published
2013Subject
Type
Collections
Metadata
Show full item recordRelated items
Showing items related by title, author, creator and subject.
-
Inhibition of Prostate Cancer Invasion by Small Molecule Antagonists of ERG and ETV1 Transcription Factors
(Georgetown University, 2013)Chromosomal translocations involving the ETS family of transcription factors are found in a majority of prostate cancers, including the most clinically aggressive forms. These translocations produce a chimeric gene, which ... -
Exploiting Structural Disorder to Enhance Small Molecule Inhibition of the Oncoprotein c-Myc DImerization with its Partner Max
(Georgetown University, 2009)The transcription factor c-Myc, in its normal function, is involved in cell cycle regulation. The uncontrolled cell proliferation consequent to c-Myc deregulation is typical of cancer and neoplastic diseases. Most known ... -
INVESTIGATION OF PROTEIN AND SMALL-MOLECULE SELF-ASSOCIATION IN THE CONTEXT OF C-MYC/MAX INHIBITION
(Georgetown University, 2016)The intrinsically disordered c-Myc oncoprotein is deregulated in ~70% of human cancers and is considered a promising but challenging drug target. To regulate transcription, c-Myc undergoes coupled folding and binding with ...
